Biotechnology: Principles and Processes
Class 12 Biology · Biology · Chapter 9
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Summary
This chapter introduces biotechnology, the use of living organisms or their components to produce useful products, and the principles and tools underlying recombinant DNA (genetic engineering) technology. The two core techniques are genetic engineering (altering DNA and introducing it into hosts) and maintaining sterile conditions for the large-scale growth of microbes and cells. Key tools of recombinant DNA technology include restriction enzymes (molecular scissors that cut DNA at specific palindromic sequences producing sticky ends), DNA ligase (molecular glue), cloning vectors (plasmids, bacteriophages) with features such as origin of replication, selectable markers and recognition sites, and host organisms. Separation and isolation of DNA fragments is achieved by gel electrophoresis. The process of recombinant DNA technology is described step by step: isolation of the gene of interest, cutting and joining DNA, amplification using the Polymerase Chain Reaction (PCR), insertion into a vector, transfer into a competent host (transformation), selection of transformants, and obtaining the foreign gene product. Methods of introducing recombinant DNA, including microinjection, biolistics (gene gun) and disarmed Agrobacterium, are covered. Bioreactors for scaling up production, downstream processing, and quality control are explained, highlighting the industrial application of these principles.
Principles of biotechnologyTools of recombinant DNA technologyRestriction enzymes and vectorsGel electrophoresis and PCRProcesses of recombinant DNA technologyBioreactors and downstream processing
Key terms
Restriction enzyme
An enzyme that recognises specific palindromic DNA sequences and cuts the DNA, used as molecular scissors.
Cloning vector
A DNA molecule (e.g., plasmid) used to carry and replicate a foreign gene inside a host cell.
PCR
Polymerase Chain Reaction, a technique to amplify a specific DNA segment in vitro using primers and Taq polymerase.
Gel electrophoresis
A technique to separate DNA fragments according to size by their migration through a gel in an electric field.
Transformation
The process of introducing recombinant DNA into a competent host cell.
Bioreactor
A vessel in which raw materials are biologically converted into specific products using microbes or cells on a large scale.
Extra questions & answers
Try one from this chapter
Restriction enzymes are often called: