GATE Biotechnology Practice Quiz

Question 1

For an enzyme that follows Michaelis-Menten kinetics, what is the initial velocity v0 when the substrate concentration [S] equals Km?

Accepted Answer

Vmax/2

Question 2

In Michaelis-Menten kinetics, the Michaelis constant Km is numerically equal to the substrate concentration at which:

Accepted Answer

v0 = Vmax/2

Question 3

An enzyme obeys Michaelis-Menten kinetics with kcat = 100 s^-1 and Km = 10 µM. If the substrate concentration is 0.1 µM, which approximation for the turnover rate per enzyme active site is most appropriate?

Accepted Answer

About 1 s^-1

Question 4

For a Michaelis-Menten enzyme, the substrate concentration is increased from 0.5Km to 2Km. By what factor does the initial velocity change?

Accepted Answer

It increases by 2-fold

Question 5

For the mechanism E + S ⇌ ES → E + P, under the Briggs-Haldane steady-state treatment, Km is given by:

Accepted Answer

(k-1 + kcat)/k1

Paper	Action	File Format
GATE Biotechnology 2010 2010
GATE Biotechnology 2011 2011
GATE Biotechnology 2012 2012
GATE Biotechnology 2013 2013
GATE Biotechnology 2014 2014
GATE Biotechnology 2015 2015
GATE Biotechnology 2016 2016
GATE Biotechnology 2017 2017
GATE Biotechnology 2018 2018
GATE Biotechnology 2019 2019
GATE Biotechnology 2020 2020
GATE Biotechnology 2021 2021
GATE Biotechnology 2022 2022
GATE Biotechnology 2023 2023
GATE Biotechnology 2024 2024