GATE Biotechnology Practice Quiz

Question 1

For an enzyme that follows Michaelis-Menten kinetics, what is the initial velocity v0 when the substrate concentration [S] equals Km?

Accepted Answer

Vmax/2

Question 2

In Michaelis-Menten kinetics, the Michaelis constant Km is numerically equal to the substrate concentration at which:

Accepted Answer

v0 = Vmax/2

Question 3

An enzyme obeys Michaelis-Menten kinetics with kcat = 100 s^-1 and Km = 10 µM. If the substrate concentration is 0.1 µM, which approximation for the turnover rate per enzyme active site is most appropriate?

Accepted Answer

About 1 s^-1

Question 4

For the mechanism E + S ⇌ ES → E + P, under the Briggs-Haldane steady-state treatment, Km is given by:

Accepted Answer

(k-1 + kcat)/k1

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