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GAT-B Syllabus
Graduate Aptitude Test - Biotechnology for admission to postgraduate programs in biotechnology.
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Students preparing for GAT-B
View all Formula of the day
Molecular Biology · PCR primers · 11 formulae in the cheatsheet
Trap of the day
A260/A280 ≈ 1.8 → pure DNA, ≈ 2.0 → pure RNA. A low ratio means protein contamination — don’t ignore it.
Revision & Exam Traps· 10 formulae · 5 traps
Biophysics · Spectrophotometry
Enzymology
Low Km = HIGH substrate affinity (Km is the [S] at ½Vmax). A low Km read as low affinity is exactly backwards.
Competitive inhibitors raise apparent Km but leave Vmax unchanged; non-competitive lower Vmax. Don’t swap them.
Population Genetics
Lab Calculations
Molecular Biology · Nucleic-acid quantitation
Molecular Biology · PCR primers
Biochemistry · Buffers
Techniques · Chromatography
Bioenergetics
Microbiology · Bacterial growth
Molecular Biology
A260/A280 ≈ 1.8 → pure DNA, ≈ 2.0 → pure RNA. A low ratio means protein contamination — don’t ignore it.
Quantitation
1 OD260 = 50 µg/mL for dsDNA, but ~40 for RNA and ~33 for ssDNA/oligos. Using 50 for all over-estimates RNA/ssDNA.
PCR
Wallace Tm = 4(G+C)+2(A+T) is valid only for SHORT oligos (<14 nt); long templates need nearest-neighbour methods.
GAT-B Syllabus (2026)
2 subjects · 12 topics
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