BET Practice Quiz

Question 1

In a double-stranded DNA molecule, the molar fraction of adenine is $0.22$. What is the molar fraction of guanine?

Accepted Answer

$0.28$

Question 2

Which one of the following statements best describes the role of dideoxynucleotides $(ddNTPs)$ in Sanger DNA sequencing?

Accepted Answer

They terminate DNA chain elongation because they lack a $3'$-$OH$ group.

Question 3

A researcher observes that an enzyme-catalyzed reaction shows the same $V_{max}$ but an increased apparent $K_m$ in the presence of an inhibitor. Which type of inhibition is most consistent with this observation?

Accepted Answer

Competitive inhibition

Question 4

During purification of a recombinant protein, the following data were obtained: total protein $=200\,\text{mg}$, total activity $=4000\,\text{units}$. After chromatography, total protein $=20\,\text{mg}$, total activity $=2000\,\text{units}$. What is the fold purification after chromatography?

Accepted Answer

$5$-fold

Question 5

A bacterial culture grows exponentially with a generation time of $30$ minutes. If the initial cell density is $2\times10^5\,\text{cells mL}^{-1}$, what will be the cell density after $2$ hours under ideal growth conditions?

Accepted Answer

$3.2\times10^6\,\text{cells mL}^{-1}$

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