Full Chapter Summary & Detailed Notes - Photosynthesis in Higher Plants Class 11 NCERT
Overview & Key Concepts
- Chapter Goal: Understand the process of photosynthesis, its significance, site, pigments, light and dark reactions, pathways (C3, C4), photorespiration, and factors affecting it. Exam Focus: Light reactions, Z-scheme, Calvin cycle, C4 vs C3. 2025 Updates: Emphasis on efficiency in arid conditions, climate impact on photosynthesis. Fun Fact: Melvin Calvin's work on carbon pathway earned Nobel in 1961. Core Idea: Photosynthesis converts light energy to chemical energy, sustaining life. Real-World: Basis for biofuels, crop yield improvement.
- Wider Scope: Links to respiration, plant growth, ecology, and global carbon cycle.
11.1 What Do We Know?
- All animals depend on plants for food; plants synthesize via photosynthesis, making them autotrophs. Heterotrophs rely on them.
- Photosynthesis: Physico-chemical process using light to synthesize organics from CO2 and H2O, releasing O2. Primary food source and atmospheric O2 provider.
- Prior knowledge: Chlorophyll, light, CO2 essential. Experiments show starch in green parts exposed to light; CO2 absorption prevents starch formation.
- Significance: Without O2, no aerobic life. Chapter covers machinery transforming light to chemical energy.
This section builds foundational understanding through school-level experiments, emphasizing empirical evidence for requirements.
11.2 Early Experiments
- Joseph Priestley (1770): Plants restore air 'damaged' by candles/mice; mint plant allows candle/mouse survival in bell jar. Hypothesis: Plants restore air removed by respiration/combustion.
- Jan Ingenhousz (1779): Sunlight essential; aquatic plants release O2 bubbles from green parts in light, not dark.
- Julius von Sachs (1854): Glucose produced/stored as starch in green parts/chloroplasts.
- T.W. Engelmann (1880s): Action spectrum using Cladophora and bacteria; O2 evolution max in blue/red light, matching chlorophyll absorption.
- Cornelius van Niel (1930s): General equation from bacterial photosynthesis; in plants, H2O is H-donor, O2 from water (confirmed by isotopes).
- Overall equation: 6CO2 + 12H2O → C6H12O6 + 6H2O + 6O2 (multistep, 12 H2O as substrate for 6 O2).
These experiments trace evolution from air purification to molecular details, highlighting O2 source and light role.
11.3 Where Does Photosynthesis Take Place?
- Primarily in green leaves, but also green stems; mesophyll chloroplasts align for max light (parallel to walls in low light, perpendicular in high).
- Chloroplast structure: Double membrane, stroma (matrix with enzymes), grana (thylakoid stacks for light reactions), stroma lamellae (connect grana).
- Division of labor: Thylakoid membrane traps light, synthesizes ATP/NADPH (light reactions). Stroma fixes CO2 to sugars (dark reactions, light-dependent via products).
- Not truly 'dark' – occur in light but not directly driven by it. Ribosomes, starch granules, lipids in stroma.
Figure 11.2 illustrates EM view; emphasizes compartmentalization for efficiency.
11.4 How Many Pigments Are Involved in Photosynthesis?
- Four pigments via chromatography: Chlorophyll a (blue-green, chief), b (yellow-green), xanthophylls (yellow), carotenoids (yellow-orange, accessory).
- Pigments absorb specific wavelengths; chlorophyll a max at blue (430 nm)/red (660 nm). Action spectrum overlaps absorption but not fully – accessory pigments broaden range.
- Accessory pigments transfer energy to chlorophyll a, protect from photo-oxidation. Photosynthesis peaks in blue/red.
Figures 11.3a-c: Absorption vs action spectra; shows efficiency beyond single pigment.
11.5 What is Light Reaction?
- Photochemical phase: Light absorption, water splitting, O2 release, ATP/NADPH formation.
- Two photosystems (PS II, PS I; discovery order, not function): LHC with antennae pigments, reaction center (P680 in PS II, P700 in PS I).
- LHC: Hundreds of pigments bound to proteins; funnel energy to reaction center chlorophyll a.
Figure 11.4: LHC diagram; enhances efficiency via wavelength diversity.
11.6 The Electron Transport
- PS II: P680 excited, e- to acceptor, downhill via cyt b6f to PS I (Z-scheme).
- PS I: P700 excited, e- to ferredoxin, then NADP+ → NADPH + H+.
- Water splitting (PS II): 2H2O → 4H+ + O2 + 4e- (oxygen evolving complex, lumen side).
- Z-scheme: Redox potential scale; explains energy flow, ATP via chemiosmosis (not detailed here).
Figure 11.5: Z-scheme; cyclic/non-cyclic photophosphorylation for ATP.
Cyclic: PS I only, e- cycle back via cyt f for ATP (no NADPH/O2). Non-cyclic: Both PS, full chain.
11.7 Where are the ATP and NADPH Used?
- Dark reactions (Calvin cycle/Biosynthetic phase) in stroma: CO2 fixation to glucose using ATP/NADPH.
- Three phases: Carboxylation (RuBP + CO2 → 3-PGA via Rubisco), reduction (3-PGA → G3P via ATP/NADPH), regeneration (RuBP via ATP).
- 6 turns: 6CO2 → C6H12O6; outputs 2 G3P for sucrose/starch.
- Rubisco: Most abundant enzyme, dual role (oxygenase in photorespiration).
C3 plants: Calvin cycle primary; efficiency issues in heat/dry.
11.8 The C4 Pathway
- Hatch-Slack pathway in tropical grasses (maize, sugarcane): Fixes CO2 to oxaloacetate (4C) in mesophyll via PEP carboxylase, then malate to bundle sheath for Calvin.
- Advantages: CO2 pump avoids photorespiration, higher efficiency in hot/dry (stomatal closure ok).
- Kranz anatomy: Mesophyll/bundle sheath dimorphic chloroplasts.
- C3 vs C4: C3 (80% plants, temperate), C4 (5%, tropical, 50% productivity).
Energy cost: C4 uses extra ATP but reduces water loss.
11.9 Photorespiration
- Rubisco oxygenase activity: RuBP + O2 → 3-PGA + phosphoglycolate (2C, toxic).
- Salvage pathway in peroxisomes/mitochondria: Glycolate → glycine → serine + CO2 (25% loss), no ATP/NADPH gain.
- Wasteful in C3 (hot/dry, high O2/CO2); absent in C4.
- Reduces productivity by 25%; evolutionary relic.
Conditions: High temp, low CO2 promote it.
11.10 Factors Affecting Photosynthesis
- Light: Saturation ~10% sunlight; blue/red effective. Too much: Damage.
- CO2: 0.03-0.04%; limiting <0.1%, saturation 10%.
- Temperature: 20-25°C optimal; enzymes denature high.
- Water: Indirect via stomatal closure.
- Blackman’s law: Limiting factor is lowest; interdependencies.
Graphs show rate vs factor; practical: Greenhouse CO2 enrichment.
Summary
- Photosynthesis: Autotrophic nutrition, light to chemical energy. Sites: Chloroplasts. Reactions: Light (thylakoid), dark (stroma). Pathways: C3, C4. Factors optimize yield.
Why This Guide Stands Out
Complete coverage: All subtopics, diagrams explained, Q&A, quiz. Exam-ready for 2025. Free & ad-free.
Key Themes & Tips
- Energy Conversion: Light → ATP/NADPH → Glucose.
- Efficiency: C4 adaptation.
- Tip: Draw Z-scheme, Calvin cycle; memorize equations.
Exam Case Studies
Compare C3/C4; explain photorespiration impact on yield.
Project & Group Ideas
- Test starch in variegated leaves; measure rate under lights.
60+ Questions & Answers - NCERT Based (Class 11)
Structured as Part A (1 mark, short answers), Part B (4 marks, ~6 lines/medium), Part C (8 marks, detailed/long). 20 per part, based on chapter, answers matching scheme.
Part A: 1 Mark Questions (Short Answers)
1. What is photosynthesis?
1 Mark Answer: Synthesis of food using light, CO2, H2O.
2. Autotrophs example?
1 Mark Answer: Green plants.
3. Priestley discovered?
1 Mark Answer: Plants restore air.
4. Ingenhousz showed?
1 Mark Answer: Sunlight essential.
5. Sachs contribution?
1 Mark Answer: Starch in chloroplasts.
6. Engelmann's method?
1 Mark Answer: Action spectrum.
7. Van Niel's inference?
1 Mark Answer: O2 from H2O.
8. Site of photosynthesis?
1 Mark Answer: Chloroplasts.
9. Chief pigment?
1 Mark Answer: Chlorophyll a.
10. Accessory pigments?
1 Mark Answer: Chl b, carotenoids.
11. PS II reaction center?
1 Mark Answer: P680.
12. PS I reaction center?
1 Mark Answer: P700.
13. Water splitting product?
1 Mark Answer: O2, H+, e-.
14. Z-scheme shape?
1 Mark Answer: Zigzag.
15. Calvin cycle site?
1 Mark Answer: Stroma.
16. Rubisco fixes?
1 Mark Answer: CO2 to 3-PGA.
17. C4 initial fixation?
1 Mark Answer: Oxaloacetate.
18. Photorespiration enzyme?
1 Mark Answer: Rubisco (oxygenase).
19. Limiting factor law?
1 Mark Answer: Blackman.
20. Optimal temp?
1 Mark Answer: 20-25°C.
Part B: 4 Marks Questions (Medium Answers ~6 Lines)
1. Why is photosynthesis important?
4 Marks Answer: Primary food source for all life; releases O2 for respiration. Converts solar energy to chemical, basis of biosphere. Without it, no aerobic life or food chain. Links to carbon cycle, climate regulation. Example: Plants as autotrophs sustain heterotrophs.
2. Describe Priestley's experiment.
4 Marks Answer: Bell jar with candle/mouse extinguishes air; mint plant restores it, allowing survival/burning. Hypothesis: Plants release O2 countering respiration/combustion. Showed air's role in growth. Limitations: Needed sunlight (later by Ingenhousz).
3. Explain action spectrum.
4 Marks Answer: Wavelengths effective for photosynthesis; peaks blue/red like chlorophyll absorption. Engelmann: Bacteria cluster in blue/red on algal spectrum. Differs from absorption (accessories fill gaps). Significance: Guides pigment studies.
4. Where does photosynthesis occur in chloroplast?
4 Marks Answer: Light: Thylakoid membrane (grana). Dark: Stroma. Mesophyll chloroplasts align for light. Double membrane, stroma lamellae connect grana. Division: Light traps energy, dark fixes CO2.
5. Role of pigments.
4 Marks Answer: Absorb light; chl a chief (reaction center), accessories (b, carotenoids) transfer energy, protect oxidation. Chromatography separates four. Broaden spectrum use. Example: Carotenoids yellow in autumn.
6. Describe light reaction.
4 Marks Answer: Photochemical: Absorbs light, splits water (O2), forms ATP/NADPH. PS I/II with LHC antennae to P700/P680. Non-cyclic: Full chain. Cyclic: ATP only. Outputs for dark phase.
7. Explain Z-scheme.
4 Marks Answer: e- flow: PS II (P680) → acceptor → cyt → PS I (P700) → ferredoxin → NADP+. Downhill redox, uphill excitations. Water splits for PS II e-. Shape: Z on potential scale.
8. Water splitting details.
4 Marks Answer: 2H2O → 4H+ + O2 + 4e- at PS II (OEC). Protons lumen, O2 stroma? Replaces PS II e-. Light-driven, no enzymes directly.
9. Cyclic photophosphorylation.
4 Marks Answer: PS I only; e- from P700 → cyt f → back via PC. No NADP/O2. ATP via chemiosmosis. When NADPH excess.
10. Calvin cycle phases.
4 Marks Answer: Carboxylation: RuBP + CO2 → 3-PGA (Rubisco). Reduction: 3-PGA + ATP/NADPH → G3P. Regeneration: G3P → RuBP (ATP). 6 cycles for glucose.
11. Role of Rubisco.
4 Marks Answer: Largest enzyme; carboxylates RuBP to 3-PGA. Also oxygenase (photorespiration). In stroma. C3 key, but inefficient hot/dry.
12. C4 pathway overview.
4 Marks Answer: Mesophyll: PEP + CO2 → OAA (4C) via PEPC. Bundle: Malate → pyruvate + CO2 for Calvin. Kranz: Ring chloroplasts. Tropical adaptation.
13. Advantages of C4.
4 Marks Answer: No photorespiration; CO2 concentrate. Efficient hot/dry, less water loss. Higher productivity (50%). Example: Sugarcane.
14. Photorespiration process.
4 Marks Answer: RuBP + O2 → 3-PGA + glycolate. Glycolate → peroxisome/myo: CO2 release, serine. 25% C loss, no ATP.
15. Factors: Light.
4 Marks Answer: Intensity/saturation curve; blue/red best. Excess: Damage PS. Limiting low light.
16. Factors: CO2.
4 Marks Answer: 0.03% atmospheric; curve rises to 0.1-1%. Limiting below, saturation high. Greenhouses enrich.
17. Blackman's law.
4 Marks Answer: Rate limited by scarcest factor. Interdependent (e.g., light/CO2). Explains plateaus.
18. Kranz anatomy.
4 Marks Answer: C4 veins: Mesophyll (granal), bundle sheath (agranal, CO2 release). Ring-like.
19. Quantum yield.
4 Marks Answer: 1 O2 per 8-10 quanta (0.125 efficiency). Measures light use.
20. Overall equation reason for 12 H2O.
4 Marks Answer: 6 O2 from 12 H2O; 6 H2O byproduct from glucose formation.
Part C: 8 Marks Questions (Detailed/Long Answers)
1. Describe early experiments on photosynthesis.
8 Marks Answer: Priestley (1770): Bell jar experiments showed plants restore 'bad air' – candle/mouse survive with mint, hypothesizing O2 release. Ingenhousz (1779): Aquatic plants bubble O2 from green parts in sunlight only, proving light/green necessity. Sachs (1854): Starch tests confirmed glucose synthesis in chloroplasts. Engelmann (1880s): Prism-split light on Cladophora with bacteria; O2 (bacteria) max blue/red, first action spectrum matching chl absorption. Van Niel (1930s): Bacterial photosynthesis (H2S donor, S product) inferred plant O2 from H2O, not CO2 – isotope proof. These built from gross to molecular understanding, leading to equation 6CO2 + 12H2O → C6H12O6 + 6O2 + 6H2O. Significance: Empirical foundation for mechanisms.
2. Explain chloroplast structure and photosynthesis sites.
8 Marks Answer: Chloroplast: Lens-shaped, double membrane, internal thylakoids (grana stacks connected by stroma lamellae) in fluid stroma (enzymes, starch). Mesophyll chloroplasts (palisade/spongy) align flat/perpendicular for light. Light reactions: Thylakoid membrane – PS I/II, e- transport, water splitting (lumen protons), ATP/NADPH. Dark reactions: Stroma – Calvin cycle (RuBP carboxylation, G3P reduction/regeneration). Figure 11.2 EM shows ribosomes, lipids. Division optimizes: Light photochemical, dark biosynthetic (light-dependent). Other sites: Green stems. Adaptations: High light – perpendicular alignment maximizes absorption.
3. Discuss pigments and spectra.
8 Marks Answer: Leaf shades from four pigments (chromatography): Chl a (blue-green, chief), b (yellow-green), xanthophylls (yellow), carotenoids (orange, accessory). Absorb specific λ; chl a max 430/660 nm (blue/red). Absorption spectrum: Pigment uptake graph. Action: Photosynthesis rate vs λ – overlaps but broader due to energy transfer from accessories to chl a. Figures 11.3: Superimposed shows blue/red peaks, minor others. Role: Accessories utilize green/yellow light, protect chl a photo-oxidation (free radicals). World’s most abundant: Chl a. Efficiency: ~1-2% solar energy fixed.
4. Elaborate light reactions with Z-scheme.
8 Marks Answer: Photochemical phase: Light absorption (LHC antennae to reaction center), water photolysis, O2 evolution, ATP/NADPH. PS II (P680): e- excited 680 nm → pheophytin → QB → cyt b6f (downhill, protons lumen for ATP). To PS I PC. PS I (P700): 700 nm excite → A0 → A1 → ferredoxin → NADP+ reductase → NADPH. Water: 2H2O → 4H+ + O2 + 4e- (OEC, Mn cluster). Z-scheme (Fig 11.5): Redox scale – excitations uphill, transports downhill; explains high energy. Photophosphorylation: Non-cyclic (full, O2/NADPH/ATP), cyclic (PS I loop, ATP only). Chemiosmosis: Proton gradient ATP synthase. Outputs fuel Calvin.
5. Describe Calvin cycle in detail.
8 Marks Answer: Biosynthetic phase (stroma): 3 stages, 6 CO2 for glucose. 1. Carboxylation: Rubisco + Mg catalyzes RuBP (5C) + CO2 → unstable 6C → 2 3-PGA (3C). 2. Reduction: 3-PGA + ATP → 1,3-bisPGA; + NADPH → G3P (glyceraldehyde-3-phosphate). 6 G3P from 6 CO2; 1 G3P exported, 5 regenerate. 3. Regeneration: G3P rearrangements (transketolase, aldolase) + 3 ATP → 3 RuBP. Net: 9 ATP + 6 NADPH per glucose. Enzymes stroma. Significance: CO2 to organics; C3 plants. Limitations: Rubisco affinity low, O2 competition.
6. Explain C4 pathway and Kranz anatomy.
8 Marks Answer: In C4 (tropical, 5% plants): Avoids photorespiration via spatial CO2 pump. Mesophyll: PEP (3C) + CO2 → OAA (4C) by PEPC (high affinity, no O2). OAA → malate/aspartate → bundle sheath. Bundle: Decarboxylation releases CO2 for Rubisco/Calvin (high [CO2]). Agranal chloroplasts (no PS II). Kranz: Vein surrounded by ring mesophyll (granal, 4C fix) + bundle sheath (agranal, Calvin). Examples: Maize, sorghum. Advantages: Hot/dry tolerance (stomata partial close), 50% higher yield. Cost: Extra 2 ATP/CO2. Vs C3: C3 temperate, photorespiration loss.
7. What is photorespiration? Why wasteful?
8 Marks Answer: C2 oxidative cycle when Rubisco oxygenase: RuBP + O2 → 1 3-PGA + 1 phosphoglycolate (2C). Glycolate peroxisome: → glyoxylate → glycine. Mitochondria: 2 glycine → serine + CO2 + NH3. Peroxisome: Serine → hydroxypyruvate → glycerate → chloroplast 3-PGA. Salvages 75% C, releases 25% CO2, consumes ATP/NADPH, no sugar. Triggers: High O2/CO2, temp >25°C, low CO2. Wasteful: Reduces C3 efficiency 25-50%, evolutionary flaw (Rubisco ancient). Absent C4/CAM. Research: Engineer Rubisco for CO2 specificity.
8. Discuss factors affecting rate.
8 Marks Answer: External: Light (intensity/duration; saturation 10% sun, blue/red max; excess bleaches), CO2 (0.03-0.04%; linear to 0.1%, plateau 10%; limiting urban), Temperature (20-25°C enzymes; high denatures Rubisco, low slows), Water (stomatal control, drought limits). Internal: Chlorophyll content, anatomy. Blackman: Rate by min factor; e.g., high light/low CO2 = CO2-limited. Graphs: S-curves. Practical: Shade crops, CO2 fertilization. Interdependence: Temp affects solubility (CO2 down high temp).
9. Differentiate C3 and C4 plants.
8 Marks Answer: C3 (80%, wheat/rice): Calvin only, mesophyll Rubisco, photorespiration high hot/dry, Kranz absent, lower hot yield. C4 (5%, maize): Hatch-Slack + Calvin, mesophyll PEPC 4C, bundle Rubisco, no photorespiration, Kranz present, higher efficiency tropics (50% productivity), extra ATP. First product: 3-PGA (C3), OAA (C4). Photorespiration: C3 yes, C4 no. Water use: C4 better. Evolution: C4 recent adaptation aridity.
10. Role of Melvin Calvin.
8 Marks Answer: Chemist (UC Berkeley), post-WWII used C14-CO2 labeling on algae to trace carbon path. Discovered RuBP cycle (Calvin-Benson), 3-PGA intermediate. Electron transfer in pigment array. Nobel 1961. Applications: Renewable energy, solar research. Bio: Minnesota PhD, radioactivity beneficial use after bombs.
11. Explain non-cyclic photophosphorylation.
8 Marks Answer: Linear e- flow: PS II absorbs 680 nm, P680* → pheophytin → plastoquinone → cyt b6f (Q-cycle protons) → plastocyanin → PS I. PS I 700 nm, P700* → A0/A1 → ferredoxin → FNR → NADP+ + H+ → NADPH. Water photolysis replaces PS II e-. ATP: Chemiosmotic, H+ gradient ATP synthase (CF1/CF0). Outputs: 1 O2, 2 NADPH, ~2.5 ATP per 2 H2O. Vs cyclic: No NADP/O2.
12. Significance of accessory pigments.
8 Marks Answer: Chl b/xanthophylls/carotenoids absorb green/yellow (chl a misses), transfer excitation energy via resonance to chl a reaction center (Förster). Broaden action spectrum, increase quantum yield. Antioxidants: Quench singlet O2, prevent chl a peroxidation (photoinhibition). Autumn colors: Chl breakdown reveals. Examples: Beta-carotene in carrots. Without: Narrow efficiency, damage high light.
13. How isotopes proved O2 source?
8 Marks Answer: Van Niel analogy: Bacteria H2S → S, not CO2. Plants: H2O18 + C16O2 → O16 (biomass/O2 from CO2? No). Ruben/Kamen: H2O18 + C16O2 → O18 in O2, C16O16 in sugar. Confirmed H2O donor, CO2 carbon. Calvin extended C14 for path.
14. Compare light and dark reactions.
8 Marks Answer: Light: Thylakoid, photochemical, direct light-driven, inputs H2O/light, outputs ATP/NADPH/O2. Dark: Stroma, biosynthetic, enzymatic (Calvin), light-dependent (uses products), inputs CO2/ATP/NADPH, outputs G3P. Not 'dark' – needs light indirectly. Coupled: Light provides reductant/energy. Location: Membrane vs matrix.
15. Impact of factors on agriculture.
8 Marks Answer: Light: Shade nets for sensitive crops. CO2: Enriched greenhouses +20% yield. Temp: Cool varieties hot areas. Water: Drip irrigation maintains stomata. Limiting: Balanced fertilizers. C4 crops (sorghum) arid-suited. Climate change: Higher temp/CO2 mixed – more photorespiration offset by CO2. Breeding: High Rubisco C3.
16. Draw and explain Z-scheme.
8 Marks Answer: [Description: Vertical redox scale, PS II left (P680), e- up to acceptor, down cyt b6f to PC, up PS I (P700), down to FD/NADP. H2O bottom, O2 up. Light arrows excitations.] Maintains potential for NADPH, protons for ATP. 2 photons/e- (one each PS). Efficiency: Serial boosts energy.
17. Why C4 evolved?
8 Marks Answer: Low CO2/high O2 atmosphere post-dinoflagellate O2 rise. Rubisco poor specificity. C4/CAM concentrate CO2, suppress oxygenase. Independent 60+ times. Cost-benefit: Extra ATP worth reduced loss/water saving. Future: Low CO2 scenarios favor.
18. Role of thylakoid membrane.
8 Marks Answer: Site light reactions: Embeds PS, carriers (PQ, cyt, PC). Impermeable H+, creates gradient (lumen acidic). Water splitting inner side. Fluidity for diffusion. Grana/stroma lamellae separate PS I/II domains. Damage: Herbicides block e- flow.
19. Quantum requirement/yield.
8 Marks Answer: Min quanta/O2 = 8-10 (yield 0.1-0.125). Emerson: Two light maxima, red enhancement. Losses: Fluorescence, heat, non-PS. Improves: Accessories. Measures health.
20. Calvin's contribution details.
8 Marks Answer: 1946-50s: Chlorella + C14-CO2, killed/time, separated compounds. First: 3-PGA (5s), then sugars. Cycle: Discovered RuBP regeneration. Bassham team. Applications: Isotope tracing in metabolism, biofuels. Legacy: Benson-Calvin cycle.
Practice Tip: Diagrams for 8 marks; time answers.